Green synthesis of copper oxide nanoparticles using Spinacia oleracea leaf extract and evaluation of biological applications: Antioxidant, antibacterial, larvicidal and biosafety assay

Copper oxide nanoparticles (CuO-NPs) recently engrossed the attention of investigators due to their incredible features and numerous applications in pharmacological industry. This research work focuses on manufacture copper an aqueous media utilizing Spinacia oleracea leaf extract. Various analytical methods like UV/Vis Spectroscopy, FT-IR spectroscopy, X-Ray diffraction studies, SEM analysis were utilized identify structure CuO produced. The formation was confirmed by band appeared at 621 cm−1 monoclinic identified patterns, which detected 2θ = 32.47°, 35.49°, 38.68°, 48.65°, 53.36°, 58.25°, 61.45°. confirms average particles around 134.8 nm size range. exhibited significant bactericidal action towards Staphylococcus aureus, Escherichia coli, proteus, klebsiella potential antifungal activity A.flavus, Rhizopus A.fumigant. Furthermore,the synthesized have larvicidal Culex. Quinquefasciatus. shows that can be a simple, environmentally friendly way has wide range uses.